The Turtles are on a visit to the zoo, so that Dr. Jane Goodfellow can give veterinary treatment to Michelangelo's pigeon friend Pete. However the other three turtles get kidnapped by robots and are caught up in a scheme by the mad Dr. Lesseau, who has plans to cross-mutate the zoo's animals to create a perfect being to conquer the world with. Only Michelangelo, Pete and Dr. Goodfellow can save them and stop the doctor's evil plan.
- Leonardo (Cam Clarke)
- Donatello (Barry Gordon)
- Michelangelo (Townsend Coleman)
- Raphael (Rob Paulsen)
- Splinter (Peter Renaday)
- April O'Neil (Renae Jacobs)
- Dr. Jane Goodfellow (Phylicia Rashad)
- Dr. Lesseau (Barry Gordon)
- Zoo Manager (Rob Paulsen)
- Robot Chimpanzees
- Robot Wolf
- Robot Bear
- Mutater Laser
- After Michelangelo sets off the island's intruder alarm, Leonardo speaks in Raphael's voice.
- When Michelangelo spots Pete rustling in the bushes, the shell on his grappling hook looks like a folded up Turtlecom.
Pop Culture references
- Dr. Lesseau and his island parodies the novel and movie of H.G. Well's The Island of Dr. Moreau.