Character voices
- Leonardo (Cam Clarke)
- Donatello (Barry Gordon)
- Michaelangelo (Townsend Coleman)
- Raphael (Rob Paulsen)
- Splinter (Peter Renaday)
- April O'Neil (Renae Jacobs)
- Dr. Jane Goodfellow (Phylicia Rashad)
- Dr. Lesseau (Barry Gordon)
Locations
- Turtles' Lair
- Central Park Zoo
- Island of Dr. Lesseau
- Island Power Plant
Vehicles
- Turtlevan
- Rowing boat
Gadgets
- Robot Chimpanzees
- Robot Wolf
- Robot Bear
- Mutater Laser
Errors
- After Michelangelo sets off the island's intruder alarm, Leonardo speaks in Raphael's voice.
- When Michaelangelo spots Pete rustling in the bushes, the shell on his grappling hook looks like a folded up Turtlecom.
Pop Culture references
- Dr. Lesseau and his island parodies the novel and movie of H.G. Well's The Island of Dr. Moreau.