General Serpiente was part of a brotherhood formed by her brothers to conquer the world. During the brotherhood's existence, Serpiente's brother Yaotl discovered a constellation known as the Stars of Kikin which would align every three-thousand years to open a portal to another dimension. During a battle between Yaotl's army and a Central American civilization, Yaotl used a wheel-like object to open the portal, but had a cost: Serpiente, along with three of her brothers were turned to stone, and thirteen monsters were summoned to Earth. The monsters quickly wiped out both Yaotl's army and his opponent. Yaotl himself was left cursed with immortality.
Three-thousand years later, Serpiente and the other stone generals were reanimated by Yaotl (under the alias of Max Winters) and Yaotl made them capture the Thirteen Monsters, assisted by the Foot Clan. They captured twelve of them and captured Leonardo as a replacement for the thirteenth monster. Later, Serpiente and her brothers led by her brother Aguila turned against Yaotl, thinking he would take their immortality for himself and liking their immortality. Yaotl, however, was trying to get rid of the immortality by casting the thirteen monsters into the portal. During the next alignment and reopening of the portal, Serpiente and the other generals revealed their intentions to summon more monsters to Earth to use as an army to finish the world conquest they had started three-thousand years ago. Serpiente claimed that the stone generals will finally have their victory. Four Turtles, led by the rescued Leonardo, then confronted the generals. During the fight, General Serpiente engaged Michelangelo in combat. The turtles defeated the stone generals and kicked them into the portal. The stone generals emerged from the portal and Aguila stated that they would never be vanquished without the thirteenth monster. Just then, the thirteenth monster barged into the room and slammed the stone generals and itself into the portal. Serpiente and the other stone generals changed back to human, apparently breaking the curse.
Serpiente's weapons are two blades that flip out from under her arms.
- Serpiente means "serpent" in Spanish.
- In the TMNT (2007) video game, she has super speed and can spit acid.