When Raphael, Michelangelo and Robin took refuge in a room in the asylum, they found themselves confronted with Isley. She attempted to kill them but found that they were just a few inches outside her reach, and she was unable to move closer because of her roots. She became agitated as the Turtles and the boy simply exited the room, remaining outside her grasp.
Though it is not shown, Isley was presumably demutated by the police after the retromutagen was found to work.
- Poison Ivy's voice actress, Tara Strong, also voices Harley Quinn in the same film.
- In the comics, she became a mutant praying mantis.