Jo and her fellow homeless mutants were originally humans who were mutated by Old Hob's mutagen bomb along with most of the other mutants confined to Mutant Town. These mutants were squatting inside the ruins of an abandoned school, and encountered Mona Lisa and Donatello as the latter two were assessing the possibility of refurbishing the school so that Mona could start teaching mutant children, as Mutant Town no longer had a functioning traditional school up to this point. Jo and her companions were initially hostile at the trespassers, but Donnie calmed the situation by promising that he could restore the rest of the building's electricity and give the squatters internet access, which could give Jo and her companions the chance of contacting their families outside the wall. Jo finally agreed that a school would be a good idea, and Donnie connected them to the internet. Later in the same issue, Jo and her companions were among many who listened to the first broadcast of Michelangelo's pirate radio station.