In the first home console video game, Splinter would sometimes appear on the pause menu giving advice. He would later be seen as a captive of the boss Mechaturtle and is rescued after the boss is defeated.
In the dialogue of the Japanese version of this game, Splinter is portrayed as April O'Neil's father.
Splinter is saved after defeating Granitor and thanks the Turtles for saving him in a brief cutscene after the battle.
Splinter appears in the opening cutscene and in the ending. In the arcade version, he appears onscreen to encourage the Turtles to fight when the player characters are idle; if the player idles too long, they will be attacked by a bomb. In the SNES port, he also hosts the 2-Player Versus mode, but his idle-animation appearance is replaced by an appearance of April O'Neil.
Splinter appears to explain the rules of the hidden minigames.
Splinter briefly pops out of a pizzeria in the first stage to give the player a pizza powerup so they have a chance to heal before the fight against Rocksteady.
He later appears in a level transition cutscene riding the Turtle Blimp.
Splinter is the penultimate captive needing to be rescued, the key to his cell guarded by Scale Tail.
Splinter is featured in this DOS computer game. Oddly, the game's incarnation of Splinter seems more based on Splinter's Mirage counterpart than on his 1987 counterpart, as he opens the game by narrating the Turtles' Mirage origin while the game itself uses characters and elements from the 1987 cartoon as well as the live-action films.
In all three versions of the game, the story mode involves Splinter being kidnapped by either Shredder (NES version) or Karai (SNES and Sega Genesis versions).
Splinter's role in the NES version is ambiguous, as his face appears upon a drone that hovers across the screen during fights and drops the fireball projectile which can be used by either combatant. After defeating Shredder at the end of Story Mode, the drone bursts open to reveal Splinter, who commends the turtles for their performance and victory.
- The manual of the Famicom version of Teenage Mutant Ninja Turtles (1989 video game) identifies April as the daughter of Splinter. This is not corroborated in any other version of the game or installment of the franchise.
- Splinter appears in six games without being captured: Teenage Mutant Hero Turtles (Grandstand handheld), Teenage Mutant Ninja Turtles: Fall of the Foot Clan, Teenage Mutant Ninja Turtles II: Back from the Sewers, Teenage Mutant Ninja Turtles: Manhattan Missions, Teenage Mutant Ninja Turtles: Turtles in Time, and Teenage Mutant Ninja Turtles: The Hyperstone Heist.
- Rescuing a captive Splinter is the ultimate end-goal of two titles: Teenage Mutant Ninja Turtles: Shredder Gets Shelled, and Sega Mega Drive/Genesis versions of Teenage Mutant Ninja Turtles: Tournament Fighters.