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Gyoji is the referee of the Battle Nexus, and a servant of the Daimyo and the Ultimate Ninja.
Gyoji first appeared in "The Ultimate Ninja", where he served as the referee for the titular ninja's mortal duel with Leonardo, while also preventing Leonardo's friends and family from intervening. After the Daimyo's arrival, he left with his employer.
He later reappeared as the referee for the entire Battle Nexus tournament, where he briefly declared the victory of the Slontags (in actuality, the Turtles) over the Gom-Tai. Once the mistake was brought to his attention, he removed the contestants from the area in order to reschedule their match. He later accompanied the Daimyo when his master met the Turtles, and was put in charge of having Leonardo's swords reforged.
He was later pulled into a rift that Drake opened above the Battle Nexus fighting arena.
He later seemingly reappeared after Leonardo and Miyamoto Usagi arrived at the Battle Nexus to beg for the Daimyo's help, and wished to aid them after the Daimyo turned against them. However, he was revealed to be the illusory form of Ultimate Drako.
The Gyoji appears in the game Teenage Mutant Ninja Turtles 2: Battle Nexus.