Dr. Cluckingsworth M.D. looks like an ordinary chicken but she has an oversized brain with green veins bulging from her head.
In "Race with the Demon", Dr. Cluckingsworth M.D. was still an ordinary chicken until she drank the mutagenic fuel sample that came from Speed Demon in Donnie's lab-barn. The mutation enlarged her brain, giving her remarkable intelligence and the ability to lay radioactive mutagen eggs. She acts as Casey's co-pilot as he races against Speed Demon.
In Return to New York, Donatello shares a goodbye towards Dr. Cluckingsworth as the Turtles leave the farm to retake New York from the Kraang.
- The Turtle Racer toy version, a.k.a. Turtle Hot Rod, has a miniature Dr. Cluckingsworth M.D. molded inside the little cage on its back.
- The cage that Dr. Cluckingsworth M.D. sits in bears a slight resemblance to R2-D2 from Star Wars.
- This is notable in that the R2 units from the Star Wars franchise serve as a fighter Pilot's assisted A.I. inside of their individual ships. Dr. Cluckingsworth M.D. serves as the Turtle Racer's A.I. and communicates with Casey via a WI-FI link from a laptop to an old Speak & Spell toy from Texas Instruments.