Buttercup is a giant bulldog mutant. Its first appearance is in the video game Teenage Mutant Ninja Turtles: Dark Horizons, where it, at first, chases the player from the right of the screen to the left. Later, it serves as the game's first boss. Buttercup sports a joint wraps, a chain, a spiked collar, and a skull motif. The cutscene at the beginning of its level shows a Lost Dog poster with traces of mutagen dotting it, implying that Buttercup was a lost dog separated from its owner before mutating.