The Boss's thugs are a group of criminals who work for the Boss in the TMHT Adventures comics.
The Turtles first encounter a pair of the Boss's thugs in Pete's Pizza Palace. While there enjoying pizza with April O'Neil, the group sees two suspicious men approach Pete. A frightened Pete tells the men that he can’t afford the prices their Boss is asking. One of the thugs informs Pete that they will have to smash his restaurant to pieces and then do the same to him. The Turtles intervene and quickly knock the men out. This is when they learn from Pete that the Boss is running a protection racket on a lot of businesses in the area. If they don’t pay them cash at the end of each week, the thugs smash their places up. Raphael asks Pete for the names of the other places being terrorized, because this sounds like their kind of job.
The next night, a pair of thugs arrive at Jack's Amusement Arcade and threaten Jack. The Turtles are nearby, playing video games. They easily handle this pair of thugs as they did the two from the previous night. Later, the Boss, one of the city’s most dangerous kingpins of crime, is fuming. In his headquarters, surrounded by his men, he says something has to be done to get those interfering ‘guys’ out of the way. As he’s pacing, the Boss has an idea. Those ‘guys’ are friends with the reporter April O’Neil. They will kidnap her and use her as a bargaining tool.
Two of his thugs arrive at April O'Neil's apartment at two a.m. and kidnap her. They leave a ransom note, which the Turtles find the next day. It instructs them to come to an abandoned warehouse. In the warehouse, nearly a dozen of the Boss's thugs wait with their boss, many with guns at the ready. The Boss tells his men to shoot at the first glimpse of the weirdos, but the Turtles come in behind them. Their surprise attack gives them time to lay into the thugs before any of them can shoot. The thugs quickly give up and drop their weapons. The Turtles locate and rescue April and then deliver all of the goons to the police.