This version of April O'Neil appears in the episode "Shredderville". She and Irma are both harem/serving girls who work at Shredder's headquarters in the Channel 6 building in this alternate world.
Both women are assigned to be the serving girls for Rocksteady and Bebop, who are human. April has been brainwashed, along with the rest of the population, into believing that mutants are evil and are the cause of the general state of disrepair in the city.
When the Turtles arrive at the Channel 6 building to enlist April's aid, she runs away from them, stating that "we don't socialize with mutants".
Later, the Turtles convince her that they are not the bad guys and are trying to rid the world of Shredder's tyranny, as well as stop the Technodrome from exploding. April talks Irma into helping them and together they take the Turtles to Shredder's office. There they discover that the Shredder is a bumbling and inept leader, who wishes that he was not in charge of the world.
When part of the building collapses and the Turtles fall through a hole in the floor, April and Irma grab onto Raphael's and Donatello's wrists and try to pull them up. April calls to Donatello to "get up, get up", but her voice fades to reveal that it is Splinter, who is trying to wake his sons from a shared dream they were having.