In New Earth Prime, April O'Neil is an ordinary lab assistant to Baxter Stockman, having no memories of or experience with the Ninja Turtles. A version of Raphael stated that it was necessary for her to "wake up" in order to start the fight against Krang.
On her way home from work, April was attacked by the Laughing Man and the Smile Clan, who had been warned about the Turtles finding her by Krang. She was then rescued by the Turtles, and after being touched by Donatello, April recognized him and apparently regained her memories. After the progenitor-Raphael was captured by Krang, April reintroduced herself to the Turtles, and stated that she knew where they had to go next. She then guided them to Oroku Saki.