Board Thread:Questions and Answers/@comment-33790467-20190814010218/@comment-995426-20191004063651

While that's possible, remember: Normal turtles have virtually no secondary sexual characteristics. If a female mutant turtle mutated under the same circumstances also has a deep voice, then it wouldn't say anything distinctive about their biological sexes, but then those females could be mistaken for male to an unknowledgeable observer. And, as we said before, Splinter may be smart and well-educated, but it's still a situation he had never been in.

You know, this is fun. It's like a logic puzzle. Anyone have more theories to test?